suppression of the nuclear factor eny2 increases insulin secretion in poorly functioning ins-1e insulinoma cells抑制核转录因子的eny2增加胰岛素分泌功能不良ins-1e胰岛瘤细胞.pdfVIP

Hindawi Publishing Corporation Experimental Diabetes Research Volume 2012, Article ID 460869, 8 pages doi:10.1155/2012/460869 Research Article Suppression of the Nuclear Factor Eny2 Increases Insulin Secretion in Poorly Functioning INS-1E Insulinoma Cells 1 1, 2 1 ¨ 1 1 2 1, 2 P. Dames, M. Weise, R. Puff, B. Goke, K. G. Parhofer, J. Seissler, and A. Lechner 1 Medizinische Klinik II, Ludwig-Maximilians-Universitat, Munchen 80336, Germany ¨ ¨ 2 Medizinische Klinik IV, Ludwig-Maximilians-Universitat, Munchen 80336, Germany ¨ ¨ Correspondence should be addressed to A. Lechner, andreas.lechner@med.uni-muenchen.de Received 29 December 2011; Revised 18 February 2012; Accepted 21 February 2012 Academic Editor: Bernard Portha Copyright © 2012 P. Dames et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Eny2, the mammalian ortholog of yeast Sus1 and drosophila E(y)2, is a nuclear factor that participates in several steps of gene transcription and in mRNA export. We had previously found that Eny2 expression changes in mouse pancreatic islets during the metabolic adaptation to pregnancy. We therefore hypothesized that the protein contributes to the regulation of islet endocrine cell function and tested this hypothesis in rat INS-1E insulinoma cells. Overexpression of Eny2 had no effect but siRNA-mediated knockdown of Eny2 resulted in markedly