内陆地区自然环境中战备消毒包内细菌同源性试验-第三军医大学学报.DOC

内陆地区自然环境中战备消毒包内细菌同源性实验研究 张 颖，王仙园，周 娟 （400038 重庆 第三军医大学护理学院野战护理教研室） 【摘 要】 目的 探讨内陆地区自然环境中战备消毒包内细菌来源。方法 根据战备消毒包内蜡样芽孢杆菌、藤黄微球菌、科氏葡萄球菌解脲亚种筛选战备仓库空气同种细菌不同菌株，提取基因组DNA，以其为模板，对16SrRNA-23SrRNA 的转录间隔区（ITS）序列进行PCR扩增，将扩增产物回收测序，将测得的基因序列用Blast进行比对。结果 蜡样芽孢杆菌、藤黄微球菌、科氏葡萄球菌解脲亚种DNA PCR扩增产物分别在850、1200、1000bp左右显现单一特异性条带；战备消毒包与空气同种细菌不同菌株之间的ITS序列相似性为98%～99%。结论 内陆地区自然环境中战备消毒包内细菌来源于空气细菌。 【关键词】 内陆地区；战备消毒包；细菌；同源性 【中图法分类号】 【文献标识码】 A Investigation on homology of bacteria in sterile packages of strategic reserve under natural environment in inland region Zhang Ying,Wang Xianyuan ,Zhou Juan (College of Nursing, Third Military Medical University, Chongqing 400038,China) 【Abstract】 Objective To investigate the origin of bacteria in sterile packages of strategic reserve under natural environment in inland region.Methods Genomic DNA from the Bacillus cereus,Micrococcus luteus and Staphylococcus cohnii ureal in sterile packages of strategic reserve and in air bacteria strains in combat readiness warehouse were extracted and used as templates to amplify the 16SrRNA-23SrRNA ITS(internal transcribed spacer)sequences by PCR. The PCR products were extracted and sequenced and sequences homology analysis was performed with BLAST.Results Amplification products of DNA PCR from Bacillus cereus,Micrococcus luteus and Staphylococcus cohnii ureal were about 800,1200,1000bp single specificity strap respectively; The 16SrRNA-23SrRNA ITS sequences of bacteria strains in sterile packages shared 98%?99% homology with which from the air bacteria strains in combat readiness warehouse.Conclusion The bacteria in sterile packages of strategic reserve came from the air in combat readiness warehouse under natural environment in inland region. 【Key words】 inland region; sterile packages of strategic reserve; bacteria; homology. 战备消毒包是战时对伤员开展救治的医疗装备，通常备用存放在自然环境中，消毒包的无菌状态是战时救治质量的重要保障。超过一定的储存时限，无菌消毒包内就会培养出细菌。了解消毒包内细菌来源，有利于采取针对性措施，确保或延长消毒包的有效储存时限。我们在内陆地区重庆市某医院战备仓库对压力蒸汽灭菌战备消毒包的储存时限进行了动态观察，对消毒包首次培养细菌进行分类，发现