睾丸皮下埋藏对精子发生影响的可逆性探讨.docVIP

睾丸皮下埋藏对精子发生影响的可逆性探讨 王达利 孙广峰 魏在荣 崔红旺 李丽摘要：目的 探讨新西兰大白兔睾丸皮下埋藏导致精子发生障碍后，将埋藏之睾丸放回正常阴囊内其精子发生障碍是否可逆。方法 育龄期雄性新西兰大白兔42只，曾有过生育史的雌性12只将雄兔随机分成组：空白组（12只）；双埋组双侧睾丸埋藏组12只）；单埋组单侧睾丸埋藏组18只）。双埋组将睾丸埋藏于两侧腹股沟区皮下；单埋组一侧睾丸置于腹股沟区皮下，另一侧睾丸保留在阴囊内；空白组不作任何处理。模型成功后空白组、双埋组、单埋组分别随机取6只进行睾丸活检均行HE染色及采用TUNEL法检测生精细胞凋亡情况空白组及双埋组6只与分别与雌兔一一配对喂养观察生育情况。同期将单埋组未取活检的12只动物未处理侧睾丸从正常阴囊内移出埋藏至同侧腹股沟区皮下，而将前期埋藏之睾丸放回刚移走睾丸的对侧正常阴囊内。单埋组睾丸放回阴囊后及分别对放回阴囊之睾丸取6只取活检，HE染色及TUNEL法检测生精细胞凋亡情况。结果 与空白组相比双埋组模型建立后HE染色见曲细精管内生精细胞明显减少，，；TUNEL法检测结果示精原细胞也有明显凋亡征象，生精细胞凋亡指数（AI）明显高于空白组（P005）；配对喂养雌兔均无生育，生育率明显低于空白组（P005）。单埋组模型建立后埋藏之睾丸活检HE染色结果双埋组相似，TUNEL法检测结果生精细胞与双埋组比较差异无统计学意义埋藏睾丸放回阴囊术后HE染色示曲细精管内生精细胞层数增多双埋组。结论 睾丸皮下埋藏与皮瓣重建阴囊一样会导致睾丸精子发生障碍，但这种损害是可逆的 关键词: 阴囊 精子发生 可逆性改变 An Reversibility Study of Burying Testis in Skin Pocket on Spermatogenesis WANG Dali, SUN Guangfeng,WEI Zai-rong et al. Department of Plastic Surgery Affiliated Hospital Zunyi Medical College , Zunyi 563003 , P.R. China Abstract：Objective To explore the reversibility of the disturbance effect of the testes were buried in thigh on spermatogenesis in New Zealand white rabbit .Methods 42 male and 12 female New Zealand white rabbits were used as the experiment animals,The male animals were divided randomly into three groups: normal control group(n=12),bilateral testes buried group(n=12), half testes buried group(n=18). In bilateral testes buried group ,a subcutaneous tunnel was dissected respectively from inner of scrotum to bilateral thigh and the testis were buried respectively to bilateral thigh. In half testes buried group, only one testis were buried to thigh ;the rabbits in normal control group were not operated. The six testes taken randomly in all groups were biopsyed at 9th week after modeling, then the testiculus were biopsied. And observed by hematoxylin-eosin staining (HE).To detect the apoptosis spermatogenic cells with TUNEL. The six animals that were not biopsied in the normal control group and the bilateral testes buried group were fed respectively with ones female rabbit