生物样本前处理技术（Biological sample pretreatment）.docVIP

生物样本前处理技术（Biological sample pretreatment） [discussion] biological sample pretreatment technology Sample pretreatment techniques play a very important role in the analysis methods, many problems can be solved through the analysis of samples, this paper will review the methods of sample and sample pretreatment process encountered before, in order to form a system. This article will be divided into 1. classification and collection of samples 2. preliminary treatment 3. separation of free drugs 4. extraction technique 5. post extraction process The five parts are expounded separately. There are many personal views, hope that the comrades can hesitate to correct me (corrected typo, correct view, discussions are welcome), and hope to learn together in the park. -------------------------------------------------------------------------------- Landlord quickly introduce it. -------------------------------------------------------------------------------- 1.1 classification of biological samples A variety of biological samples include plasma, serum, whole blood, lymph, saliva, tissues, hair, urine, bile, tears, spinal fluid, sweat, milk, amniotic fluid, feces, and exhaled gases. The following text is mainly from in vivo drug analysis, edited by Yao Tongwei in 2001. Biological samples can be roughly divided into Uniform samples: blood, urine, saliva, bile, cerebrospinal fluid, lymph, milk, and gonadal secretions; Heterogeneous samples: heart, liver, spleen, lung, kidney, stomach, intestine, reproductive organs, brain, body fat, thymus, adrenal gland and skeletal muscle and other 11 kinds of organs and tissues. Ruth Endacott[1] summarizes the problems that should be paid attention to in the collection of clinical samples so as to make the sampling adequate and applicable. [1]: Endacott, R, Botti, M., Clinical, research, 3:, sample, selection., Intensive, Crit, Care, Nurs., 2005, Feb; 21 (): 51-5. 1.2 biological sample collection 1.2.1 blood sample 1.2.1.1 composition Blood sampl