七氟醚预处理对大鼠肾缺血再灌注损伤影响.pdf

EffectsofSevoflurane onrenal preconditioning inrats ischemia-reperfusioninjury Shu PostgraduateJinjun Director Xiaoni Prof．Zhang Prof．LinCaizhu Abstract objeetive：To theeffectsofsevoflurane onrenal investigate pretreatment inratsandrelatedmechanismsofheatshock 70． ischemia-reperfusioninjury proteins adultmale ratswere dividedinto Methods：Twenty--fourSprague·-Dawleyrandomly three asimilar withoutocclusionof groups(n=8)．(1)Shamgroup：Performoperation renalblood the of renalischemia- flow．(2)URgroup：Performoperationpartial of for60minute reperfusion(I／R)．(3)SEV MAC)sevoflurane group：inhalation2．2％(1 andwashoutfor10minuteswere beforeI／R．After60minutesofischemia performed and120minutesof ClevelsandMDAconcentrations reperfusion，serumBUN，Cr,Cys weremeasured．Thesliceswerealsoobservedunder andelectron kidney microscope theSalTle used for time，we microscope．At immuno·histochemistrystainingmeasuring ofliSP70 of tissues． expression kidney Results：1．There differenceintheserum levelofthe werenoobvious BUN，Cr three theSelXllTlClevela