Historical Development of Embryo Culture Media胚胎培养液的历史发展.pptVIP

Let the Embryos Choose(Assayed by abolishing the 2-cell block) Lawitts JA, Biggers JD (1991) Optimization of mouse embryo culture media using simplex methods. J Reprod Fertil 91, 543-556. (Medium SOM) Lawitts JA, Biggers JD (1993) Culture of preimplantation embryos. Methods Enzymol 225, 153-164. (Medium KSOM) Two media initially developed for the culture of mouse preimplantation embryos G1/G2. (Based on the toxicological studies and the“Back to nature principle”) Gardner DK, Lane M (1993) Amino acids and ammonium production regulate mouse embryo development in culture. Biol Reprod 48, 377-385 KSOMAA. (Based on the “Let the embryos choose principle”.) Biggers JD, McGinnis LK, Raffin M (2000) Amino acids and preimplantation development of the mouse in protein –free potassium simplex optimized medium. Biol Reprod 63, 281-293. Culture Human Preimplantation Embryos to the Blastocyst G1/G2 Requires a two-step protocol using two media of different composition sequentially. Gardner DK, Lane M (1997) Culture and selection of viable human blastocysts: a feasible proposition for human IVF. Hum Reprod Uptake 3, 367-382. KSOMAA Requires a one-step using a single medium. Biggers JD, Racowsky C (2002) The development of fertilized human ova to the blastocyst stage in KSOMAA: is a two-step protocol necessary? Reprod Biomed Online 5, 133-140. One-step culture KSOMAA Two-step culture DEVELOPMENT OF HUMAN ZYGOTES TO THE BLASTOCYST AFTER FIVE DAYS IN CULTURE P-1/CCM Biggers JD, Racowsky C (2002) The development of fertilized human ova to the blastocyst stage in medium KSOMAA: Is a two-step protocol necessary? Reprod Biomed Online 5, 133-140. 51/82 (62.2%) 81/140 (57.9%) Some Comments The introduction of G1/G2 led to at least six other commercially available two step protocols for the culture of human preimplantation embryos. Several newer versions of G1/G2 have been developed. KSOMAA is the basis of Global? which can be used as a one