长期饲喂钬对鲤鱼非特异性免疫酶活性的影响-生态毒理学报.pdfVIP

２０１６年　 第 １１卷 生 态 毒 理 学 报 Vol. 11, 2016 第 １期ꎬ３７５￣３８１ Asian Journal of Ecotoxicology No.1, 375￣381 DOI: 10.7524/AJE.1673￣5897.20151105002 吴红松. 长期饲喂钬对鲤鱼非特异性免疫酶活性的影响[J]. 生态毒理学报ꎬ2016, 11(1): 375￣381 Wu H S. Effect of holmium on the activities of nonspecific immune enzymes in Cyprinus carp io [J]. Asian Journal of Ecotoxicology, 2016, 11(1): 375￣ 381 (in Chinese) 长期饲喂钬对鲤鱼非特异性免疫酶活性的影响 吴红松* 菏泽学院ꎬ菏泽274015 : : 收稿日期 2015￣11￣05 　 　 录用日期 2015￣12￣24 摘要:以鲤鱼为研究对象ꎬ进行为期60 d 的饲喂试验ꎬ研究了硝酸钬对其非特异性免疫的影响ꎮ 结果表明:与对照组相比ꎬ在 实验剂量范围内ꎬ超氧化物歧化酶(SOD) 、过氧化氢酶(CAT) 、谷胱甘肽过氧化物酶(GSH￣Px)在肝脏中呈先升高后降低的趋势ꎬ SOD 、GSH￣Px 酶活性在20 mg kg￣1 时ꎬ升高显著(P0.05) ꎬ三者在65 mg kg￣1 时都被显著抑制(P0.05) ꎻ在肾中都被诱导ꎬ在65 mg kg￣1 升高显著(P0.05) ꎮ 肝肾中丙二醛(MDA)含量都变化不显著(P0.05) ꎮ 抗超氧阴离子自由基、抑制羟自由基能力和单 胺氧化酶(MAO)活性都降低ꎬ且在65 mg kg￣1 时降低显著(P0.05 或P0.01) ꎮ 因此ꎬ在饲料中添加稀土元素钬20 、42 、65 mg kg￣1 ꎬ能够影响非特异性免疫力ꎬ且鲤鱼肝组织比肾脏反应更敏感ꎮ 关键词:钬ꎻ鲤鱼ꎻ非特异性免疫 文章编号:1673￣5897 (2016 )1￣375￣07　 　 中图分类号:X 171.5　 　 文献标识码:A Ｅｆｆｅｃｔ ｏｆ Ｈｏｌｍｉｕｍ ｏｎ ｔｈｅ Ａｃｔｉｖｉｔｉｅｓ ｏｆ Ｎｏｎｓｐｅｃｉｆｉｃ Ｉｍｍｕｎｅ Ｅｎｚｙｍｅｓ ｉｎ Ｃｙｐｒｉｎｕｓ ｃａｒｐｉｏ * Wu Hongsong Heze University, Heze 274015, China Ｒｅｃｅｉｖｅｄ 5 November 2015　 　 ａｃｃｅｐｔｅｄ24 December 2015 Ａｂｓｔｒａｃｔ:In order to investigate the effects of nitrate holmium on innate immune response of Cyprinus carp io (C. carp io), a 60￣day period of feeding trial was conducted. The result showed that, with the increasing of nitrate hol￣ mium, the activities of catalase (CAT), the superoxide dismutase (SOD) and the glutathione peroxidase (GSH￣Px) in the liver were all increased first and then decreased. In the liver, the activities of SOD and GSH￣Px were induced significantly when the concentration of nitrate holmium was 20 mg kg￣1(P 0.05), while the three enzymes men￣ tioned above were significantly inhibited in the group including 65 mg kg￣1 nitrate