神经营养因子受体TrKB对人FOB第三军医大学学报.DOC

RanBP9的表达对骨肉瘤细胞增殖、凋亡及转移的影响﹡ 张樨1，吕杨帆1， 戴欢子2，孟刚3，郭乔楠1（400037，重庆，第三军医大学，1 新桥医院病理科, 2 大坪医院肾内科，3 西南医院病理科） 【摘要】 目的 研究RanBP9（Ran-binding protein 9）在骨肉瘤细胞的表达情况和对骨肉瘤细胞增殖、凋亡及转移的影响，探讨RanBP9在骨肉瘤细胞生物学功能中发挥的作用。方法 Real-time PCR、免疫荧光细胞染色和检测的mRNA和蛋白水平SaOS2细胞模型并检测低表达RanBP9对骨肉瘤细胞SaOS2细胞在低级别来源的骨肉瘤细胞系MG63中的表达要高于高级别来源的细胞系SOS2和U2OSP0.05）；稳定低表达的SaOS2细胞（si）细胞与其对照细胞siCON-SaOS2（siCON）相比P0.05），而凋亡率下降，有显著差异（P0.05）。的表达抑制了骨肉瘤细胞的增殖和转移能力，促进了骨肉瘤细胞的凋亡Supported by the General Program of National Natural Science Foundation of China,[通讯作者] 郭乔楠，e-mail：qiaonan85@263.net The effect of RanBP9 on proliferation，apoptosis and metastasis in human osteosarcoma 【Abstract】 Objective To explore the effect of RanBP9 on proliferation，apoptosis and metastasis in human osteosarcoma, so as to discuss its biology functions in osteosarcoma. Methods Real-time PCR, immunofluorescence and Western-blot to examine RanBP9 mRNA and protein expression levels in osteosarcoma cell lines; we have performed a series of biological tests using RanBP9 was down-regulated during the osteosarcoma SaOS2 cell line. Results We found that RanBP9 is highly expressed in human osteosarcoma cell lines and RanBP9 expression in MG63 cell line（derived from low-grade osteosarcoma）was significant higher (p0.05) than in U2OS or SaOS2 cell lines (derived from high-grade osteosarcoma). Immunofluorescence and Western-blot also showed the same result as the Real-time PCR .Low expression of RanBP9 in SaOS2 cell line not only markedly increased (p0.05) cell vitality and growth, colony formation, but also stimulated migration and invasion .Consistently, down-regulated RanBP9 was associated with reduced (p0.05) basic apoptosis assayed by annexin APC-PI. Conclusion RanBP9 increased apoptosis and reduced proliferation, as well as reduced the metastasis .Taken together,the study that suggests RanBP9 has a potent tumor-suppressor role likely in osteosarcoma. 【Key words】 RanBP9; osteosarcoma; proliferation; apoptosis ; metastasis RanBP9，又名RanBPM（