结核菌分子检验技术之简介与原理.PDF

結核菌分子檢驗技術之簡介與原理 The Techniques for Molecular Detection of Mycobacterium tuberculosis 全國結核病實驗室品質監測 及人員認證計畫 Topics  Introduction of Mycobacterium tuberculosis  Nucleic acids basic structure  General methods for DNA extraction  Comparison of different DNA extraction for M. tuberculosis  Verification or Validation of FDA-approved tests and laboratory-developed tests for Infectious Diseases  Physical and Chemical Methods for Nucleic Acid Detection  Nucleic Acid Amplification Technologies  結核病分子生物檢驗技術最新發展現況(CDC提供) Mycobacterium tuberculosis  Mycobacterium tuberculosis (MTB) is a pathogenic bacterial species in the family Mycobacteriaceae and the causative agent of most cases of tuberculosis (TB).  M. tuberculosis has an unusual, M. tuberculosis bacterial colonies waxy coating on its cell surface (primarily mycolic acid) and acid- fast detection techniques are used.  The genome of the H37Rv strain was published in 1998. Its size is 4 million base pairs, with 3959 genes. M. tuberculosis visualization using the Ziehl–Neelsen stain (acid-fast stain) /wiki/Mycobacterium_tuberculosis Cell wall of M. tuberculosis Schematic diagram of Mycobacterial cell wall. 1. outer lipids 2. mycolic acid 3. polysaccharides (arabinogalactan) 4. peptidogl