hrp(辣根过氧化物酶)标记抗体的方法（HRP (horseradish peroxidase) labeled antibody method）.doc

hrp(辣根过氧化物酶)标记抗体的方法（HRP (horseradish peroxidase) labeled antibody method） The enzyme labeled antibody labeled antibody HRP principle and method, two glutaraldehyde method and sodium periodate method of basic medical immunology experimental guide [] Source: Medical basic immunology experiment guidance, editor in chief Jin Boquan, Li Enshan, first edition. Beijing: World Book Publishing House, 1990 Enzyme markers include enzyme labeled antigens, enzyme labeled antibodies, and enzyme labeled SPA. The quality of enzyme markers is directly related to the quality The success of the enzyme immunoassay system is therefore known as the critical reagent. Enzyme labeling is the most commonly used enzyme marker The utility model is formed by connecting an enzyme and a specific antibody by proper methods. The quality of enzyme labeled antibody mainly depends on its purity, Enzymes and antibodies with strong activity and high affinity, and secondly, have good preparation methods. At present, high quality enzymes (such as horseradish peroxide) are present Chemical enzyme (abbreviated as HRP) has been supplied in china. High quality antibodies can be obtained by extraction and purification. In preparation The method is simple and easy to operate, with high yield, no binding activity and no interfering substance. (1) enzyme preparations and their substrates Enzymes that are non-toxic and exhibit colored chemical reactions can be used in principle as markers. But used as a marker antibody The enzyme should meet the following requirements: (1) easy to source and easy to purify; (2) high specific activity and stable property; (3) enzyme activity and amount of energy Measured by a simple method. Horseradish peroxidase (HRP) and alkaline phosphatase are commonly used in the enzyme immunoassay technology (AP) followed by glucose oxidase, beta galactosidase, lysozyme, and malate dehydrogenase. Because Horseradish Peroxidase (HRP) is higher than the activity, stable, small molecular weight,