玉米-大豆轮作及氮肥施用对土壤细菌群落结构的影响-作物学报.pdfVIP

作物学报 ACTA AGRONOMICA SINICA 2013, 39(11): 2016−2022 / ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2013.02016 玉米-大豆轮作及氮肥施用对土壤细菌群落结构的影响 周 岚1,2,** 杨 永1,** 王占海3 陈 阜1 曾昭海1,* 1 , 100193; 2 , 132101; 3 , 162650 : 3 (050 100 kg hm−2)(--)-I ( --)-II (--), PCR-DGGE - , , 3 (100 kg hm−2) - II , - I -I , -II , 3 , , : ; ; ; ; DGGE; Influence of Maize-Soybean Rotation and N Fertilizer on Bacterial Community Composition 1,2,** 1,** 3 1 1,* ZHOU Lan , YANG Yong , WANG Zhan-Hai , CHEN Fu , and ZENG Zhao-Hai 1 College of Agriculture and Biotechnology, Beijing 100193, China; 2 Jilin Agricultural Science and Technology College, Jilin 132101, China; 3 Hulunbeier Agricultural Institute, Zhalantun 162650, China Abstract: In soybean flowering period, took rhizosphere soil samples from three treatments including continuous cropping soy- bean (soybean-soybean-soybean), maize-soybean rotation I (soybean-maize-soybean) and maize-soybean rotation II (maize- maize-soybean). Each treatment had three levels of nitrogen application including 0, 50, and 100 kg ha−1. Soil microbial commu- nity changes under maize-soybean rotation system and various nitrogen applications were investigated by the techniques of dena- turing gradient gel electrophoresis (DGGE) analysis. The results showed that the biological diversity and abundance of three kin