about cryosurgical t739 mice la795 subcutaneous the transplanted tumor necrosis and apoptosis（关于cryosurgical la795 t739小鼠皮下移植肿瘤坏死和凋亡）.docVIP

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2017-09-05 发布于浙江
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about cryosurgical t739 mice la795 subcutaneous the transplanted tumor necrosis and apoptosis（关于cryosurgical la795 t739小鼠皮下移植肿瘤坏死和凋亡）.doc

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about cryosurgical t739 mice la795 subcutaneous the transplanted tumor necrosis and apoptosis（关于cryosurgical la795 t739小鼠皮下移植肿瘤坏死和凋亡）

About cryosurgical T739 mice LA795 subcutaneous the transplanted tumor necrosis and apoptosis Author: Wen Ju uranium segment Yun, Zou said Kun, Fenghua Song, Nie Zhoushan [Abstract] Objective: To study the cryoablation ablation T739 mouse lung adenocarcinoma LA795 subcutaneously transplanted tumor necrosis and apoptosis. The evaluation cryocare frozen the feasibility and efficacy of the treatment of non-small cell lung cancer. Methods: T739 mice LA795 lung adenocarcinoma animal model of subcutaneously transplanted tumor after cryoablation, the the optical microscope frozen necrosis ablation law, end labeling (TUNEL) in situ detection of surrounding frozen injury zone apoptosis, immunohistochemical method to check Bc1 2 and Bax in Western Blot ice hockey the surrounding freezing damage zone Caspase 3 expression and poly adenosine diphosphate nucleoside polymerase (PARP cleavage activity results: in the the frozen central area of #8203;#8203;the cell death to necrosis, necrosis presents “double peak effect: cryosurgical to appear 1st necrosis peak 3 h after surgery, the necrotic area / area of #8203;#8203;the tumor (47 + -5.51%, and 4 d after 2nd necrosis peak necrotic area / area of #8203;#8203;the tumor (68 + -4.69% in the the surrounding freezing damage zone, morphological examination by light microscopy and TUNEL staining can be observed in clear cell apoptosis, reached a peak of 8 ~ 16 h after apoptosis, apoptosis rate (71 + -6.41 %. Immunohistochemistry showed expression of Bcl-2 protein expression in no significant change before and after freezing, bax protein in frozen upregulation Western Blot results: frozen hockey peripheral damage zone Caspase 3 and PARP cut, activate Conclusion: The cryosurgical by necrosis and apoptosis are two ways to achieve effective ablation and cryoablation may by Caspase pathway induced peripheral damage zone with apoptosis mechanism of apoptosis Bax protein, Bcl 2 unrelated. [Keywords:] Endocare argon-helium knife, cry