about the role of laminin and type iv collagen in cultured nerve cells of the spinal cord neurite outgrowth（层粘连蛋白的作用和iv型胶原培养神经细胞脊髓神经突的产物）.docVIP

About the role of laminin and type IV collagen in cultured nerve cells of the spinal cord neurite outgrowth Author: Han Yan, LU Kai, Yang Li, Zhang Hui [Keywords] laminin [Abstract] AIM: To detect the influence of laminin and type IV collagen on the axon growth of the cultured spinal neuron. METHODS: Laminin and collagen IV were used in the experimental group and laminin and collagen IV combined with associated antibody, collagen I and polylysine were used in the control groups. Spinal neuron cells of the same density were cultured in each group. Each group was stained by the immunochemical method of Neuron-specific Enolase (NSE) and the length of the neuron axons was measured by the computer image system. RESULTS: The spinal neuron cells and their axons were stained by NSE. The axons length of the laminin and collagen IV groups was longer than that of other groups but no significant difference was observed. CONCLUSION: Laminin and collagen IV can stimulate the axon growth of spinal neuron cells. [Keywords] laminin, collagen, Neurons [Abstract] Objective: To observe laminin and type IV collagen role in neurite outgrowth of spinal cord nerve cells under in vitro culture conditions. Methods: laminin and collagen type IV bag culture plates to combine corresponding antibody plates coated plates of type I collagen and polylysine control, the culture of spinal cord cells added to the plates at the same concentration. cultured cells in each group after 5 d neuron specific enolase enzyme (NSE immunohistochemical staining using computer image analysis techniques groups neurite length and analyzed statistically. Results: Immunohistochemical staining is visible in each group have more nerve cells and their neurite outgrowth, statistical analysis results table BenQ laminin group and type IV collagen group neurite growth length no significant difference in the laminin Group neurite growth length is longer than the other groups. collagen type IV group neuron axon