about rhucd40l on human colon cancer transplanted into nude mice the treatment of（关于rhucd40l人类结肠癌移植到裸体小鼠的治疗）.docVIP

About rhuCD40L on human colon cancer transplanted into nude mice the treatment of Author: ZHU Xiao-Kun Mu Xiangming, when North Korea Zhou Guangjun [Abstract] Objective: To study in vivo experiments confirmed rhuCD40L the treatment of human colon cancer, which further explore the anti-tumor mechanism. Methods: subcutaneous inoculation of human colon cancer HT-29 human colon carcinoma cell model established after the 20 nude mice were randomly divided into two groups, the treatment group to rhuCD40L for subcutaneous injection, subcutaneous injection of normal saline as the control group, regularly observed in nude mice living conditions and measured their body weight, mice were sacrificed 5 weeks after treatment and take the tumor, measuring tumor size and tumor weight groups, and compare the results. Results: All 20 tumor formation in nude mice, all mice in the experiment were in good living conditions, no one died in nude mice, the control group and treatment group between tumor size and tumor weight differences, the difference was statistically significant (P lt;0.05. Conclusion: rhuCD40L on human colon cancer have a therapeutic effect. [Keywords] CD40, CD40 ligand, synthetic trimeric CD40L, nude mice, colon cancer CD40L on the expression of CD40 in tumor cells with a wide range of growth regulation. A large number of studies have shown that, CD40L and CD40 expression in the tumor can directly inhibit the interaction of a variety of tumor growth. Colon cancer is a common malignancy in the gastrointestinal tract, in China in recent 20 years, especially in large cities, morbidity and mortality have increased year after year. the treatment of colon cancer is still surgical resection is the preferred treatment, but because of local recurrence and metastasis rate was higher after 5-year survival rate is still is low. The current need to find a better means of adjuvant therapy to improve survival in patients with colon cancer, so this e