about the role of platelet-derived growth factor on human retinal pigment epithelial cells（血小板源生长因子的作用对人类视网膜色素上皮细胞）.docVIP

About the role of platelet-derived growth factor on human retinal pigment epithelial cells Author: Secretary the Walkers benefits sickness Hanquan Hong Du Hongjun [Keywords] growth factor Keywords: growth factor, platelet-derived; human retinal pigment epithelial cells; proliferation; transitional Abstract: Objective To observe the platelet-derived growth factor (PDGF on human retinal pigment epithelial cells (hRPE proliferation and migration ability. Vitro 4 to 6 human retinal pigment epithelial cells into DMEM group (modified Eagle medium liquid containing 20mL · L-1 calf serum DMEM group (20g · L-1 DMEM, each group with different concentrations (0.1,1,5,10,50 and 100μg · L-1 of PDGF, and then by MTT observed colorimetric analysis of its role in the proliferation of hRPE; application of the the hRPE injury model counting cells into the defect area, quantitative observation of PDGF on the migration of hRPE Results ① proliferative effect: of 0.1 ~~ 100μg · L-1 of PDGF can promote promoting proliferation proliferation of hRPE of cells, DMEM group 10μg · L-1, the most obvious, the proliferation rate of 153%; 20g · L-1 the DMEM group of 50 ~ 100μg · L-1 proliferation was 174%, the strongest effect. ② transitional role: PDGF significantly promote hRPE transitional, and a dose-dependent manner, 50 ~ 100μg · L-1 to promote transitional strongest DMEM group migration rate was 510%, 20 mL · L-1 FBS + DMEM group of the 640 % Conclusion PDGF can promote the proliferation and migration of hRPE have serum can strengthen that role, suggesting that it may play an important role in the pathogenesis of PVR. Keywords: platelet-derived growth factor; human retinal pig-ment epithelium cells; proliferation; migration Abstract: AIM To investigate the effects of platelet-derived growth factor on the proliferation and migration of cultured human retinal pigment epithelium cells (hRPE.METHODS Cultured hRPE of the4 ~ 6th passage were devided into two groups DMEM (Delbecco’s