青蒿琥酯对表皮鳞癌A431细胞增殖和凋亡的影响.docVIP

青蒿琥酯对表皮鳞癌A431细胞增殖和凋亡的影响 江忠勇,梅 峰,于 彬,韩 鹃,田衍平,李世峰,周德山 (第三军医大学基础部组胚教研室,重庆 400038) 摘 要： 目的 初步探讨青蒿琥酯(Artesunate, ART)对表皮鳞癌细胞生长和凋亡的影响及其相关机制。方法 以人表皮鳞癌A431细胞系和永生化角质形成细胞HaCaT细胞系为研究对象，顺铂（DDP）为阳性对照，通过MTT法和流式细胞术观察ART对A431和HaCaT细胞增殖和凋亡的影响和去铁敏（DFOM）的阻断效果。结果 ART对癌细胞具有较明显的杀伤作用, 而对永生化的角质形成细胞的损害显著低于DDP。ART能够诱导A431凋亡，其凋亡率为（19.87±0.03）%，加入DFOM后，ART诱导的肿瘤细胞凋亡率下降至（7.37±0.02）%。流式细胞术检测细胞周期发现ART主要使A431积聚于S期[(67.6 ±4.12)%, P0.05]。结论 ART可能通过诱导癌细胞A431凋亡发挥抑制其生长的作用，且在一定范围内与药物剂量呈正相关，这种作用与Fe2+介导有关。因ART毒副作用明显低于DDP，所以，可能更具临床应用前景。 关键词：青蒿琥酯；皮肤癌；A431；细胞凋亡 作者简介；江忠勇，男，重庆市人，硕士，主要从事肿瘤发生机制研究。电话；（023 通讯作者；周德山，电话（023email: dszhou2000@ The proliferation and apoptosis of epidermal Squamous cell lines A431 affected by Artesunate JIANG zhong-yong,MEI feng ,YUN bin, HAN juan, TIAN yan-ping，LI shi-feng，ZHOU de-shan（Department of Histology and Embryology, College of Medicine, Third Military Medical University,Chongqing 400038, China） Abstract: Objevect To investigate the effects that growth and apoptosis of epidermal carcinoma cell affected by Artesunate (ART) and its mechanism. Methods: The growth inhibition, apoptosis and cell cycles of A431 (a cell line of malignant epidermal carcinoma) and HaCaT (a normal keratinocyte cell line) were detected with MTT and FCM. Cisplatin (DDP) was as positive control. DFOM(deferoxamine mesylate) was used to block the role of ART. Results : ART could inhibit A431 growth, sem-inhibition ratio is 60umol/L. The results showed that ART could partly induce A431 cell apoptosis, the ratio of apoptosis （19.87±0.03）%, but pretreatment with the DFOM (60umol/L), the ratio of apoptosis was decresed to （7.37±0.02）%; Comparing with DDP, the ART was low toxicant for HaCaT. The cell cycle was measured by FCM and the A431 cells were mainly stopped to S phase reaching to （67.6±4.12）% (P0.05,compared with control). Conclusion : ART is low toxicant and high performance for epidermal carcinoma cell A431. The apoptosis induced by ART was partly depended on Fe2+. Our results ind