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烟实夜蛾信息素结合蛋白3cDNA的克隆,序列分析与原核表达
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Cloning and sequencing of cDNA encoding
pheromone binding protein 3 from the
Helicoverpa assulta (Guenée) and its
Article · anuary 2006
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Xiaoguang Liu Shiheng An
College of Plant Protection Henan Agriculture University
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Yuan Guo-hui
Henan Agriculture University
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Acta Entomologica Sinica, October 2006, 49(5):733-739 ISSN 0454-6296
3 cDNA 、
*
刘晓光, 安世恒, 罗梅浩 , 郭线茹, 原国辉
(, 450002)
:RT-PCR Helicoverpa assulta (Hass) 3 (Hass
PBP3)。 , 495 bp, 164 , 18.5 kD。
N-22 。 , 142 , 16.1 kD,
5.44。 , PBP3 ,
。 pGEX-4T-2 。 IPTG 、SDSWestern
, PBP3 BL21 , 42 kD ,
。
:;;;
:Q966 :A :0454-6296(2006)05-0733-07
Cloning and sequencing of cDNA encoding pheromone binding protein 3
from the Helicoverpa assulta (Guenée)and its expression in Escherichia coli
*
LIU Xiao-Guang, AN Shi-Heng, LUO Mei-Hao , GUO Xian-Ru, YUAN Guo-Hu
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