一篇典型的响应曲面法优化培养基的文献.pdfVIP

Process Biochemistry 40 (2005) 681–690 Direct fermentation of starch to l(+) lactic acid in SSF by Lactobacillus amylophilus GV6 using wheat bran as support and substrate: medium optimization using RSM B.J. Naveena a , Md. Altaf a , K. Bhadrayyab , S.S. Madhavendra c , Gopal Reddy a,∗ a Department of Microbiology, Osmania University, Hyderabad 500007, AP, India b Swaroop Tech. Consultancy, Secunderabad 500003, AP, India c Indian Institute of Chemical Technology (IICT), Hyderabad 500007, AP, India Received 6 November 2003; received in revised form 2 January 2004; accepted 30 January 2004 Abstract Wheat bran is an underexploited cheap byproduct obtained during the milling process of wheat. The ability of Lactobacillus amylophilus GV6 to hydrolyze raw starch in wheat bran to produce l(+) lactic acid was studied in solid state fermentation (SSF), opening a novel method for the utilization of these agricultural byproducts. l(+) Lactic acid production by L. amylophilus has been reported by various groups from processed and unprocessed starch but there are no reports available from natural starches in SSF. All amylolytic wild strains reported so far have high yield efficiencies at low substrate concentrations whereas at high substrate concentrations lactic acid production was low. L. amylophilus GV6 showed high yield efficiency at high substrate concentration in SSF. To improve and optimize l(+) lactic acid production by L. amylophilus GV6, response surface methodology (RSM) using central composite rotatable design was adopted in SSF. MINITAB-13 was used for planning the experiments, data analysis, contour diagrams and response optimizations. The optimum media composition was obtained