固相萃取-高效液相色谱-二极管阵列检测法同时测定饮料中10种合成着色剂.pdfVIP

现代食品科技 Modern Food Science and Technology 2013, Vol.29, No.6 固相萃取-高效液相色谱-二极管阵列检测法同时 测定饮料中10种合成着色剂 咸瑞卿，王小兵，李启艳，石峰，李俊婕 （山东省食品药品检验所，山东济南250101 ） 摘要：本文建立了固相萃取-高效液相色谱-二极管阵列检测法（SPE-HPLC-PDA ）同时测定饮料中10种合成着色剂（新红、柠檬 黄、苋菜红、靛蓝、胭脂红、日落黄、诱惑红、亮蓝、赤藓红、酸性橙Ⅱ）的含量。样品经Waters Oasis WAX 固相萃取柱净化，采用 Aglient TC-C18色谱柱（5 μm，4.6 mm×250 mm），以甲醇和0.02 mol/L的乙酸铵溶液为流动相,梯度洗脱，PDA检测波长为210 nm~650 nm 。以保留时间结合待测物的紫外吸收光谱进行定性分析，采用外标法进行定量分析。10种合成色素线性范围为1~20 μg/mL(r0.999)； 方法检出限为0.01~0.02 mg/kg；加标回收率为90.2~100.6% ，RSDs 为0.44~2.48% （n=6 ）。该方法简单、准确、灵敏，适用于饮料中合 成着色剂的测定。 关键词：固相萃取；高效液相色谱；合成着色剂；二极管阵列；饮料 文章篇号：1673-9078(2013)6-1413-1416 Simultaneous Determination of 10 Synthetic Colors in Drink by Solid-Phase Extraction Coupled with HPLC/Photodiode Array Detection XIAN Rui-qing, WANG Xiao-bing, LI Qi-yan, SHI Feng, LI Jun-jie (Shandong Institute for Food and Drug Control, Ji’nan, 250101) Abstract: A method for simultaneous determination of 10 synthetic colors (new red, tartrazine, amaranth, indigotin, carmine red, sunset yellow, allura red, brilliant blue, acid orange Ⅱ and erythrosine) in drink by solid-phase extraction coupled with high performance liquid chromatography -photodiode array detector was developed. The drink samples were cleaned up by Waters Oasis WAX and separated on an Aglient TC-C18 (5 μm, 250 mm×4.60 mm) column with methanol-0.02 mol/L ammonium acetate as mobile phase by gradient elution. The wave lengths for detection were from 210 nm to 650 nm. Qualitative analysis of synthetic colors was based on their retention times and ultraviolet absorption spectra. External standard method was applied for quantitative analysis. The results showed that the linear ranges of 10 synthetic colors were 1~20 μg/mL (r ＞0.999). The recoveries