宁肠汤对肝郁脾虚型肠易激综合征大鼠血清SS、5-HT影响地实验研究.pdf

6．血清5-羟色胺（5-HT）含量测定····································12 7．结肠黏膜病理组织学观察··········································12 三、分析与讨论·························································13 （一）中医学对IBS 的认识··············································13 1．IBS 的中医归属··················································13 2．病因病机························································13 3．辨证分型及治疗··················································14 （二）现代医学对IBS的认识·············································15 1．对IBS 本质的认识················································15 2．病因与发病机制··················································15 3．治疗····························································15 （三）肝郁脾虚型IBS模型大鼠的评价·····································16 （四）西药对照组药物选择··············································17 （五）宁肠汤组方分析··················································18 （六）宁肠汤对肝郁脾虚型IBS 大鼠生长抑素（SS）的影响··················20 （七）宁肠汤对肝郁脾虚型IBS大鼠5-羟色胺（5-HT）的影响·················20 （八）问题与展望······················································21 四、结论·······························································22 参考文献·······························································23 附图···································································27 致谢···································································29 文献综述·······························································30 万方数据 中文摘要 宁肠汤对肝郁脾虚型肠易激综合征大鼠血清 SS、5-HT 影响的实验研究 目的：观察心理应激诱导的肝郁脾虚型肠易激综合征大鼠血清SS 和5-HT 的变化， 探讨肠易激综合征的发病机制及宁肠汤治疗肠易激综合征的机理。 方法：选用健康清洁级雄性SD 大鼠72 只，体重180～200g，以慢性束缚+适度夹尾 刺激作为心理应激源诱导IBS 模型。造模成功后将实验大鼠随机分为正常对照组，模型 对照组，得舒特组，宁肠汤低、中、高剂量组，各组分别灌胃给药，每日 1 次，连续14 天。灌胃剂量按照实验动物研究 “等效剂量”的计算方法，各组大鼠