重庆地区儿童诺如病毒基因型别特点研究-第三军医大学学报.DOCVIP

重庆地区儿童诺如病毒基因型别特点研究 廖炀1，陈军华彭哲幸琳琳张爱华朱启曾玫复旦大学附属儿科医院dr.liaoyang@。 摘要：目的：了解重庆地区儿童诺如病毒基因型别特点。方法：收集2008年8月至2009年7月全年轮状病毒抗原阴性的急性非细菌性腹泻标本，用real-time RT-PCR方法扩增诺如病毒ORF1-ORF2链接区，测序扩增片段后进行比对并分型及构建系统进化树。结果：全年获得806份标本，共检测出251份为诺如病毒阳性标本，其中GⅠ组4例，GⅡ组247例。已分型的诺如病毒GⅡ基因组（108例）以GII.4/2006b占绝对优势，其构成比达62.96%（68/108），其次为GII.3占25.93%（28/108）；GII.6占6.48%(7/108)；GII.2占1.85%(2/108)；此外还分别检测出GII.7、GII.12、GII.13各1例(1/108)。结论？ 关键词：诺如病毒；基因分型；婴幼儿；分子流行病学；RT-PCR The characterization of norovirus genotypes in children in Chongqing Liao yang1, Chen Junhua1, Peng Zhe1, Xing Lin-lin1, Zhang Ai-hua1, Zhu Chao-min 1, Liu Zuo-yi 1, Zhu Qi-rong 2, Zheng mei2 (1. Department of Gastroenterology in the Childrens Hospital of Chongqing Medical University, , Chongqing, 400014; 2. Department of infectious diseases in Childrens Hospital of Fudan University, Shanghai, 201102) Corresponding author: Zhu Chao-min, Liu Zuo-yi Abstract: Objective: To investigate norovirus genotype characteristics of children in Chongqing. Methods: stool specimens of nonbacterial acute diarrhea with rotavirus antigen-negative were collected from August 2008 to July 2009. real-time RT-PCR was used to detect the ORF1-ORF2 junction regions of norovirus. the sequencing fragments of RT-PCR products were blasted in GenBank and classified. phylogenetic trees were constructed by the neighbor-joining method. Results: 251 of 806 stool specimens were detected for the norovirus-positive, of which 4cases belong to GⅠ group and 247, GⅡ group. Among the sub-genotypes of GⅡ group (108 cases had been genotyped successfully), GII.4/2006b is dominant, constituent ratio is 62.96% (68/108), and GII.3 account for 25.93%（28/108）, GII.6 6.48%(7/108), GII.2 1.85%(2/108), respectively. We also detected GII.7, GII.12,and GII.13. Key words: norovirus; genotype; infant; molecular epidemiology; RT-PCR 诺如病毒( Norovirus, NV)是引起急性非细菌性胃肠炎的重要病原之一，诺如病毒感染性腹泻在全世界范围内均有流行[1-2]。1995年我国报道了首例诺如病毒感染，其后全国各地相继发生多起诺如病毒感染性腹泻暴发疫情。既往的研究表明不同国家、同一国家不同地区感染率都有所不同[3]。重庆地区对诺如病毒的研究资料比较缺乏。本研究调查了重庆地