a framework for white blood cell segmentation in microscopic blood images using digital image processing一个框架,用于微观血液中白细胞分割图像使用数字图像处理.pdfVIP

A Framework for White Blood Cell Segmentation in Microscopic Blood Images Using Digital Image Processing Farnoosh Sadeghian, Zainina Seman, Abdul Rahman Ramli, Badrul Hisham Abdul Kahar, and M-Iqbal Saripan Abstract Evaluation of blood smear is a commonly clinical test these days. Most of the time, the hematologists are interested on white blood cells (WBCs) only. Digital image processing techniques can help them in their analysis and diagnosis. For example, disease like acute leukemia is detected based on the amount and con- dition of the WBC. The main objective of this paper is to segment the WBC to its two dominant ele- ments: nucleus and cytoplasm. The segmentation is conducted using a proposed segmentation framework that consists of an integration of several digital image processing algorithms. Twenty micro- scopic blood images were tested, and the proposed framework managed to obtain 92% accuracy for nu- cleus segmentation and 78% for cytoplasm segmentation. The results indicate that the proposed framework is able to extract the nucleus and cytoplasm region in a WBC image sample. Keywords: White blood cell segmentation, Active contours, Snake algorithm, Zack thresholding. 1. Introduction White blood cells (WBC) or leukocytes play a significant role in the diagnosis of different diseases, and therefore, extracting infor- mation about that is valuable for hematologists. In the past, dig- ital image processing techniques have helped to analyze the cells that lead to more accurate, standard, and remote disease diagnosis systems. However, there are a few complications in extracting the data from WBC due to wide variation of cells in shape, size, edge, and position. Moreover, si