a full-length enriched cdna library and expressed sequence tag analysis of the parasitic weed, striga hermonthica全身的丰富cdna图书馆和表达序列标签的分析寄生杂草,striga hermonthica.pdfVIP

Yoshida et al. BMC Plant Biology 2010, 10:55 /1471-2229/10/55 R E S E A R C H A R T I C L E Open Access Research article A full-length enriched cDNA library and expressed sequence tag analysis of the parasitic weed, Striga hermonthica 1 1,2 3 3 2 1 Satoko Yoshida , Juliane K Ishida , Nasrein M Kamal , Abdelbagi M Ali , Shigetou Namba and Ken Shirasu* Abstract Background: The obligate parasitic plant witchweed (Striga hermonthica) infects major cereal crops such as sorghum, maize, and millet, and is the most devastating weed pest in Africa. An understanding of the nature of its parasitism would contribute to the development of more sophisticated management methods. However, the molecular and genomic resources currently available for the study of S. hermonthica are limited. Results: We constructed a full-length enriched cDNA library of S. hermonthica, sequenced 37,710 clones from the library, and obtained 67,814 expressed sequence tag (EST) sequences. The ESTs were assembled into 17,317 unigenes that included 10,319 contigs and 6,818 singletons. The S. hermonthica unigene dataset was subjected to a comparative analysis with other plant genomes or ESTs. Approximately 80% of the unigenes have homologs in other dicotyledonous plants including Arabidopsis, poplar, and grape. We found that 589 unigenes are conserved in the hemiparasitic Triphysaria species but not in other plant species. These are good candidates for genes specifically involved in plant parasitism. Furthermore, we found 1,445 putative simple sequence repeats (SSRs) in the S. hermonthica unigene dataset. We tested 64 pairs of PCR primers flanking the SSRs to develop genetic markers for the detection of polymorphisms. Most primer sets