a h2o2 biosensor based on immobilization of horseradishperoxidase in a gelatine network matrix基于固定的过氧化氢生物传感器的horseradishperoxidase明胶网络矩阵.pdfVIP

Sensors 2005, 5, 277-283 sensors ISSN 1424-8220 © 2005 by MDPI /sensors A H O Biosensor Based on Immobilization of Horseradish 2 2 Peroxidase in a Gelatine Network Matrix Hui Yao, Nan Li, Yan-Li Wei and Jun-Jie Zhu* Key Lab. of Analytical Chemistry for Life Science, Department of Chemistry, Nanjing University, Nanjing 210093, P.R. China *To whom correspondence should be addressed; Email: jjzhu@; Tel Fax: (+86)-25 Received: 8 June 2004 / Accepted: 14 November 2004 / Published: 4 May 2005 Abstract: A simple and promising H O biosensor has been developed by successful 2 2 entrapment of horseradish peroxidase (HRP) in a gelatine matrix which was cross-linked with formaldehyde. The large microscopic surface area and porous morphology of the gelatine matrix lead to high enzyme loading and the enzyme entrapped in this matrix can retain its bioactivity. This biosensor exhibited a fast amperometric response to hydrogen peroxide (H O ). The linear range for H O determination was from 2.5×10-5 to 2 2 2 2 2.5×10-3 M, with a detection limit of 2.0×10-6 M based on S / N = 3. This biosensor possessed very good reproducibility. Keywords: H O biosensor, horseradish peroxidase, gelatine matrix. 2 2 Introduction During the past few decades, nume