a high resolution map of a cyanobacterial transcriptome蓝藻的高分辨率的地图的转录组.pdfVIP

A high resolution map of a cyanobacterial transcriptome Vijayan et al. Vijayan et al. Genome Biology 2011, 12:R47 /2011/12/5/R47 (25 May 2011) Vijayan et al. Genome Biology 2011, 12:R47 /2011/12/5/R47 RESEARCH Open Access A high resolution map of a cyanobacterial transcriptome Vikram Vijayan 1,2, Isha H Jain2 and Erin K O’Shea1,2* Abstract Background: Previous molecular and mechanistic studies have identified several principles of prokaryotic transcription, but less is known about the global transcriptional architecture of bacterial genomes. Here we perform a comprehensive study of a cyanobacterial transcriptome, that of Synechococcus elongatus PCC 7942, generated by combining three high-resolution data sets: RNA sequencing, tiling expression microarrays, and RNA polymerase chromatin immunoprecipitation sequencing. Results: We report absolute transcript levels, operon identification, and high-resolution mapping of 5’ and 3’ ends of transcripts. We identify several interesting features at promoters, within transcripts and in terminators relating to transcription initiation, elongation, and termination. Furthermore, we identify many putative non-coding transcripts. Conclusions: We provide a global analysis of a cyanobacterial transcriptome. Our results uncover insights that reinforce and extend the current views of bacterial transcription. Background The unicellular cyanobacterium S. elongatus PCC 7942 Over the past few decades considerable progress has been is a genetically tractable model organism for prokaryotic made in understanding the mechanisms and regulation of photosynt