a highly sensitive enzyme-amplified immunosensor based on a nanoporous niobium oxide (nb2o5) electrode一个高度敏感的enzyme-amplified免疫传感器基于纳米多孔氧化铌(nb2o5)电极.pdfVIP

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a highly sensitive enzyme-amplified immunosensor based on a nanoporous niobium oxide (nb2o5) electrode一个高度敏感的enzyme-amplified免疫传感器基于纳米多孔氧化铌(nb2o5)电极.pdf

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a highly sensitive enzyme-amplified immunosensor based on a nanoporous niobium oxide (nb2o5) electrode一个高度敏感的enzyme-amplified免疫传感器基于纳米多孔氧化铌(nb2o5)电极

Sensors 2010, 10, 5160-5170; doi:10.3390/s100505160 OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Article A Highly Sensitive Enzyme-Amplified Immunosensor Based on a Nanoporous Niobium Oxide (Nb O ) Electrode 2 5 1 1 2 2 2 Chang-Soo Lee , Dohyoung Kwon , Jeng Eun Yoo , Byung Gun Lee , Jinsub Choi and Bong Hyun Chung 1,* 1 BioNanotechnology Research Center (BNRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-333, Korea; E-Mails: cslee@kribb.re.kr (C.-S.L.); kwondh@kribb.re.kr (D.-H.K.) 2 Department of Chemical Engineering, Inha University, 253 Yonghyun-Dong, Nam-Gu, Incheon 402-751, Korea; E-Mails: overstep2@ (J.-E.Y.); byungdali@ (B.-G.L.); jinsub@inha.ac.kr (J.C.) * Author to whom correspondence should be addressed; E-Mail: chungbh@kribb.re.kr; Tel.: +82-42-860-4442; Fax: +82-42-879-8594. Received: 26 March 2010; in revised form: 13 April 2010 / Accepted: 10 May 2010 / Published: 25 May 2010  Abstract: We report on the development of an enzyme-amplified sandwich-type immunosensor based on a thin gold film sputtered on an anodic nanoporous niobium oxide (Au@Nb O ) electrode. The electrocatalytic activity of enzymatically amplified 2 5 electroactive species and a stable electrode consisting of Au@Nb O were