a new triterpene from uncaria macrophylla and its antitumor activity钩藤的新三萜烯、其抗肿瘤活性.pdfVIP

Molecules 2012, 17, 1883-1889; doi:10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article A New Triterpene From Uncaria macrophylla and Its Antitumor Activity Guangli Sun 1,2, Xiaopo Zhang 1, Xudong Xu 1,*, Junshan Yang 1, Mingliang Zhong 1 and Jingquan Yuan 1,3 1 Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences Peking Union Medical College, Beijing 100193, China; E-Mails: guanglisun@126.com (G.S.); xiaopozhang2011@126.com (X.Z.); junshanyang@ (J.Y.); mingliangzhongsky@163.com (M.Z.) ; yjqgx@163.com (J.Y.) 2 Department of Pharmacy, School of Pharmacy, Hebei United University, Tangshan, Hebei 063000, China 3 Guangxi Institute of Medicinal Plant Development, Nanning, Guangxi 530023, China * Author to whom correspondence should be addressed; E-Mail: xdxu@; Tel./Fax: +86-010-5783-3296. Received: 10 January 2012; in revised form: 3 February 2012 / Accepted: 7 February 2012 / Published: 14 February 2012 Abstract: On our ongoing investigation, a new oleanolic triterpene, 3β,6β,19α-trihydroxy- 12-oleanen-28-oic acid (1) was obtained from the chloroform-soluble portion of the 90% alcohol-water extract of the stem bark of Uncaria macrophylla. Its structure was elucidated by extensive spectroscopic methods, including 1D and 2D (1 1 H- H COSY, HSQC and HMBC) NMR and HR-ESI-MS. The cytotoxicities of the compound was evaluated against two cancer cell lines of MCF-7 and HepG2 by the MTT method,