a phenotypic screening platform to identify small molecule modulators of chlamydomonas reinhardtii growth, motility and photosynthesis确定小分子表型筛选平台调节器的衣藻reinhardtii增长,能动性和光合作用.pdfVIP

Alfred et al. Genome Biology 2012, 13:R105 /2012/13/11/R105 METHOD Open Access A phenotypic screening platform to identify small molecule modulators of Chlamydomonas reinhardtii growth, motility and photosynthesis 1,2,3 1,2,4 1 1 1 1,6 5 Simon E Alfred , Anuradha Surendra , Chris Le , Ken Lin , Alexander Mok , Iain M Wallace , Michael Proctor , Malene L Urbanus1,4, Guri Giaever1,3,4 and Corey Nislow1,2,3* Abstract Chemical biology, the interfacial discipline of using small molecules as probes to investigate biology, is a powerful approach of developing specific, rapidly acting tools that can be applied across organisms. The single-celled alga Chlamydomonas reinhardtii is an excellent model system because of its photosynthetic ability, cilia-related motility and simple genetics. We report the results of an automated fitness screen of 5,445 small molecules and subsequent assays on motility/phototaxis and photosynthesis. Cheminformatic analysis revealed active core structures and was used to construct a naïve Bayes model that successfully predicts algal bioactive compounds. Background components of its light perception [8] and light response Chemical biology uses small molecules to study and complexes [9]. Evolutionary studies have focused on evo- manipulate biological systems (reviewed in [1]). By alter- lution of fitness under selective pressures (elevated [CO2], ing an organisms’ normal state, and thereby affecting sexual/asexual populations) and the evolution of multicel- growth or development, we can learn about the contribu- lularity [10-13]. Ch