a sandwich hiv p24 amperometric immunosensor based on a direct gold electroplating-modified electrode三明治hiv p24基于直接黄金electroplating-modified电极的电流型免疫传感器.pdfVIP

Molecules 2012, 17, 5988-6000; doi:10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article A Sandwich HIV p24 Amperometric Immunosensor Based on a Direct Gold Electroplating-Modified Electrode Lei Zheng 1,†, Liyong Jia 1,2,†, Bo Li 1, Bo Situ 1, Qinlan Liu 1, Qian Wang 1,* and Ning Gan 2,* 1 Clinical Laboratory Center, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China; E-Mails:nfyyzl@163.com (L.Z.); jialiyong@126.com (L.J.); boli198777@163.com (B.L.); drstb@126.com (B.S.); liuqinlan1206@163.com (Q.L.) 2 The State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Material Science and Chemical Engineering of Ningbo University, Ningbo 315211, China † These authors contributed equally to this work. * Authors to whom correspondence should be addressed; E-Mails: wangqian@ (Q.W.); ganning@ (N.G.); Tel./Fax: +86-20-6164-1007 (Q.W.); Tel.: +86-574-8760-9933 (N.G.); Fax: +86-574-8760-0734 (N.G.). Received: 8 March 2012; in revised form: 25 April 2012 / Accepted: 9 May 2012 / Published: 18 May 2012 Abstract: Acquired immune deficiency syndrome (AIDS) is a severe communicable immune deficiency disease caused by the human immune deficiency virus (HIV). The analysis laboratory diagnosis of HIV infection is a crucial aspect of controlling AIDS. The p24 antigen, the HIV-1 capsid protein, is of considerable diagnostic interest because it is detectable several days earlier than host-generated HIV antibodies f