a potentiometric indirect uric acid sensor based on zno nanoflakes and immobilized uricase电位间接尿酸传感器基于氧化锌nanoflakes和固定化尿酸酶.pdfVIP

Sensors 2012, 12, 2787-2797; doi:10.3390/s120302787 OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Article A Potentiometric Indirect Uric Acid Sensor Based on ZnO Nanoflakes and Immobilized Uricase Syed M. Usman Ali 1,2,*, Zafar Hussain Ibupoto 1, Muhammad Kashif 3, Uda Hashim 3 and 1 Magnus Willander 1 Department of Science and Technology, Linköping University, Campus Norrköping, Norrkoping SE-60174, Sweden; E-Mails: zafar.hussain.ibupoto@liu.se (Z.H.I.); magwi@itn.liu.se (M.W.) 2 Department of Electronic Engineering, NED University of Engineering and Technology, Karachi 75270, Pakistan 3 Nano Biochip Research Group, Institute of Nano Electronic Engineering (INEE), University Malaysia Perlis, Kangar, Perlis 01000, Malaysia; E-Mails: kashif_bme@ (M.K.); uda@.my (U.H.) * Authors to whom correspondence should be addressed; E-Mail: syeal@itn.liu.se or uashah68@.pk; Tel.: +46-11-363-119; Fax: +46-11-363-270. Received: 25 December 2011; in revised form: 9 February 2012 / Accepted: 1 March 2012 / Published: 1 March 2012 Abstract: In the present work zinc oxide nanoflakes (ZnO-NF) structures with a wall thickness around 50 to 100 nm were synthesized on a gold coated glass substrate using a low temperature hydrothermal method. The enzyme uricase was electrostatically immobilized in conjunction with Nafion membrane on the surface of well oriented ZnO-NFs, resulting in a sensitive, selective, stable and reproducible uric acid sensor. The electroch