a new method for the determination of nucleic acid using an eu3+– nicotinic acid complex as a resonance light scattering probe核酸测定的新方法使用一个欧盟三国+ -烟酸复杂的共振光散射探针.pdfVIP

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a new method for the determination of nucleic acid using an eu3+– nicotinic acid complex as a resonance light scattering probe核酸测定的新方法使用一个欧盟三国+ -烟酸复杂的共振光散射探针.pdf

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anewmethodforthedeterminationofnucleicacidusinganeu3–nicotinicacidcomplexasaresonancelightscatteringprobe核酸测定的新方法使用一个欧盟三国-烟酸复杂的共振光散射探针

Molecules 2009, 14, 10-18; doi:10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article A New Method for the Determination of Nucleic Acid Using an Eu3+– nicotinic Acid Complex as a Resonance Light Scattering Probe Meng Guo 1,2, Lin-Tong Wang 1, Xia Wu 2, Wei Xu 2 and Jing-He Yang 2,* 1 Microscale Science Institute, Department of Chemistry, Weifang University, Weifang 261061, P.R. China. E-mail:guomeng7101@126.com (M. G.) 2 Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, P.R. China * Author to whom correspondence should be addressed; E-mail: yjh@; Tel.: +86-0536-8785283; Fax: +86-0531 Received: 24 November 2008; in revised form: 13 December 2008 / Accepted: 16 December 2008 / Published: 23 December 2008 Abstract: This study found that in Tris-HCl buffer, the resonance light scattering (RLS) intensity of the Eu3+-nicotinic acid system can be greatly enhanced by nucleic acids and the enhanced intensity is proportional to the concentration of nucleic acid in the range of -8 -5 -1 -8 -1 7×10 -1×10 g·mL for fsDNA, and its detection limit is 2×10 g·mL . Based on this, a new method for the determination of nucleic acids is proposed. Synthetic and actual samples are determined satisfactorily. The interaction mechanism