a potentiometric formaldehyde biosensor based on immobilization of alcohol oxidase on acryloxysuccinimide-modified acrylic microspheres一个电位甲醛生物传感器基于固定化酒精氧化酶acryloxysuccinimide-modified丙烯酸微球.pdfVIP

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a potentiometric formaldehyde biosensor based on immobilization of alcohol oxidase on acryloxysuccinimide-modified acrylic microspheres一个电位甲醛生物传感器基于固定化酒精氧化酶acryloxysuccinimide-modified丙烯酸微球.pdf

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a potentiometric formaldehyde biosensor based on immobilization of alcohol oxidase on acryloxysuccinimide-modified acrylic microspheres一个电位甲醛生物传感器基于固定化酒精氧化酶acryloxysuccinimide-modified丙烯酸微球

Sensors 2010, 10, 9963-9981; doi: 10.3390/s101109963 OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Article A Potentiometric Formaldehyde Biosensor Based on Immobilization of Alcohol Oxidase on Acryloxysuccinimide-modified Acrylic Microspheres Yew Pei Ling and Lee Yook Heng * School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia (UKM), 43600 Bangi, Selangor Darul Ehsan, Malaysia; E-Mail: peiling840917@ * Author to whom correspondence should be addressed: E-Mail: leeyookheng@yahoo.co.uk; Tel.: +60-3 Fax: +60-3 Received: 11 October 2010; in revised form: 19 September 2010 / Accepted: 25 September 2010 / Published: 5 November 2010 Abstract: A new alcohol oxidase (AOX) enzyme-based formaldehyde biosensor based on acrylic microspheres has been developed. Hydrophobic poly(n-butyl acrylate-N-acryloxy- succinimide) [poly(nBA-NAS)] microspheres, an enzyme immobilization matrix, was synthesized using photopolymerization in an emulsion form. AOX-poly(nBA-NAS) microspheres were deposited on a pH transducer made from a layer of photocured and self-plasticized polyacrylate membrane with an entrapped pH ionophore coated on a Ag/AgCl screen printed electrode (SPE). Oxidation of formaldehyde by the immobilized AOX resulted in the production of protons, which can be determined via the pH transducer. Effects of buffer concentrations, pH and different amount of immobilization matrix