四球茶转录组 SSR 位点信息分析.PDFVIP

安徽农业大学学报, 2017, 44(4): 558-562 Journal of Anhui Agricultural University [DOI] 10.13610/ki.1672-352x001 网络出版时间：2017/8/11 11:53:57 [URL] /kcms/detail/34.1162.S1153.002.html 四球茶转录组 SSR 位点信息分析 黎瑞源，邢 辉，申 铁 (贵州师范大学贵州省信息与计算科学重点实验室，贵阳 550001) 摘 要：对基于高通量测序拼接获得的99 741 条四球茶嫩叶Unigenes 进行了SSR （simple sequence repeats） 位点分析，共揭示了23 732 个SSR 位点，分布于 18 552 条Unigenes 中，SSR 发生频率为23.79% ；含有SSR 位点 的Unigenes 的总长度为226 188 bp ，SSR 位点间的平均距离为 1/2.07 kb，重复长度平均长度为9.53 bp 。在四球茶 转录组SSR 中，二核苷酸重复和三核苷酸重复是主要的重复类型，分别占总SSRs 的47.53%和25.92% ；在181 种 重复基元类型中，优势重复基元分别是AG/CT 、A/T 、ACC/GGT ，分别占总SSRs 的41.38% 、22.10%和6.49%， 共占总SSRs 的比例达69.97% 。 关键词：四球茶；转录组；SSR 中图分类号：S571.1 文献标识码：A 文章编号：1672352X (2017)04055805 Analysis of SSR information in Camellia tetracocca Zhang transcriptome LI Ruiyuan, XING Hui, SHEN Tie (Key Laboratory of Information and Computing Science Guizhou Province ，Guizhou Normal University，Guiyang 550001) Abstract: In this study, a total of 99 741 unigenes were de novo assembled based on the bud transcriptome se- quencing of Camellia tetracocca Zhang. Totally, 23 732 SSRs were detected in 18 552 unigenes, accounting for 23.79% of all the unigenes. The total length of unigenes containing SSR loci was 226 158 bp. The mean distance between SSRs was 1/2.07 kb and the mean length of the repeat motif was 9.53 bp. Di-nucleotide repeats were the most abundant followed by tri-nucleotide repeats, making up about 47.53% and 25.92% of the total SSRs, respec- tively. Among the 181 repeat motifs, AG/CT, A/T and ACC/GGT were the most frequent motifs in di-, mono- and tri-nucleotide repeats, accounted for 41.38%, 22.10% and 6.49%, respectively, and which totally accounted for 69.97% of the t