galactosemia rat lens pathological changes（半乳糖血症大鼠晶状体病理变化）.docVIP

Galactosemia rat lens pathological changes Author: Yanjing Xia, Planter, Zhao Yufang [Keywords] galactosemia [Abstract] AIM: To observe the pathological changes in the lens of hypergalactosemia mice. METHODS: By intraperitoneal injection of galactose, the hypergalactosemia mice models were induced. The lens of the mice were enucleated and were observed by light microscopy and electron microscopy in the early stage of the disease. RESULTS: On the third day of hypergalactosemia induction, many vacuoles were observed in the epithelial cells of the lens by light microscopy, and the fibers were also observed to have been swollen. The electron microscopic examination revealed that the gap junctions were destroyed on membranes of the epithelial cells of the lens and many vacuoles were observed in the plasma of the cells. CONCLUSION: Hypergalactosemia at the early period can cause lens opacity and finally lead to cataract. [Keywords] galactosemia; lens crystalline / pathology; rats [Abstract] Objective: application of optical microscopy and electron microscopy studies, the The galactosemia pups incidence of early pathological changes of the lens to explore the process of the lens structural changes in the high galactosemia pathogenesis. Methods: Wistar rat pups using intraperitoneal injection of galactose induced high galactosemia, taken in the early stages of onset lens, respectively, application of the change of the optical microscope and an electron microscope lens structure results: Wistar rats rats after high galactosemia induced 3 d light microscope can see the lens epithelial cells in cell vacuoles, fiber swelling electron microscope, cell membrane gap junction abnormalities visible intracytoplasmic vacuolar structure. Conclusions: The incidence of high galactosemia in early stage can lead to lens opacity, and ultimately lead to cataracts. [Keywords] galactose hyperlipidemia; crystal / pathology, rats 0 Introduction High galactosemia is caused