glial cell line-derived neurotrophic factor protect parkinson model rat substantia nigra dopaminergic neurons mechanism analysis（神经胶质细胞line-derived神经营养因子保护帕金森模型大鼠黑质多巴胺能神经元机制分析）.docVIP

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2017-09-06 发布于浙江
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glial cell line-derived neurotrophic factor protect parkinson model rat substantia nigra dopaminergic neurons mechanism analysis（神经胶质细胞line-derived神经营养因子保护帕金森模型大鼠黑质多巴胺能神经元机制分析）.doc

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glial cell line-derived neurotrophic factor protect parkinson model rat substantia nigra dopaminergic neurons mechanism analysis（神经胶质细胞line-derived神经营养因子保护帕金森模型大鼠黑质多巴胺能神经元机制分析）

Glial cell line-derived neurotrophic factor protect Parkinson model rat substantia nigra dopaminergic neurons mechanism analysis Author: Ding Yanxia, #8203;#8203;LIU Hong-Mei, Wang Hongjun, Wang Yanjiang, high Dianshuai [Keywords] dopaminergic neurons Abstract: Objective To study the glial cell line-derived neurotrophic factor (glial cell line-derived neurotrophic factor, GDNF protect dopaminergic (dopamine, DA neurons process, the calcium-binding protein D28K (calbindin28K, CB and astrocytes the possible role of interstitial cells. Methods right striatum of rats injection of 6 - hydroxydopamine (6-OHDA) Preparation of Parkinson’s disease (Parkinson disease, PD model injection after the 7th day, based on behavior analysis screening model mouse, model mice were randomly divided into three groups: control group, PBS group (the right side of the substantia nigra injection of PBS and GDNF group (right substantia nigra injections of GDNF the blank control animals, respectively, in 6-OHDA injection after 7 days, 14 days substantia nigra, 21 days and 28 days, the PBS group and GDNF group animals in the first 14 days after the 6-OHDA injection, respectively, 21 and 28 days, line segments continuous coronal paraffin, tyrosine hydroxylase (tyrosine hydroxylase , TH, CB and glial fibrillary acidic protein (glial fibrous acid protein of GFAP immunohistochemistry staining, respectively DA neurons, including the CB neurons and astrocytes, light microscopy and cell count, statistically processing results ① TH immunohistochemical staining results :6-OHDA injection after 28 days, the expression of the right side of the GDNF group rat substantia nigra TH positive neurons was significantly more than the control group and PBS group; ② CB immunohistochemical staining results: CB positive neurons seven days after injection of 6-OHDA, the right side of the blank control group rats substantia nigra can still be observed, after the point in time of the control group and the PBS group