breakdown of resistance to grapevine downy mildew upon limited deployment of a resistant variety分解的抗葡萄霜霉病抗性品种的限制部署.pdfVIP

Peressotti et al. BMC Plant Biology 2010, 10:147 /1471-2229/10/147 RESEARCH ARTICLE Open Access Breakdown of resistance to grapevine downy mildew upon limited deployment of a resistant variety 1,4 1,2 3 4,6 4,5 Elisa Peressotti , Sabine Wiedemann-Merdinoglu , François Delmotte , Diana Bellin , Gabriele Di Gaspero , Raffaele Testolin4,5, Didier Merdinoglu1,2, Pere Mestre1,2* Abstract Background: Natural disease resistance is a cost-effective and environmentally friendly way of controlling plant disease. Breeding programmes need to make sure that the resistance deployed is effective and durable. Grapevine downy mildew, caused by the Oomycete Plasmopara viticola, affects viticulture and it is controlled with pesticides. Downy mildew resistant grapevine varieties are a promising strategy to control the disease, but their use is currently restricted to very limited acreages. The arising of resistance-breaking isolates under such restricted deployment of resistant varieties would provide valuable information to design breeding strategies for the deployment of resistance genes over large acreages whilst reducing the risks of the resistance being defeated. The observation of heavy downy mildew symptoms on a plant of the resistant variety Bianca, whose resistance is conferred by a major gene, provided us with a putative example of emergence of a resistance-breaking isolate in the interaction between grapevine and P. viticola. Results: In this paper we describe the emergence of a P. viticola isolate (isolate SL) that specifically overcomes Rpv3, the major resistance gene carried by Bianca at chromosome 18. We show that isolate SL has the same behaviour as two P. viticola isol