comparison of germinal center markers cd10, bcl6 and human germinal center-associated lymphoma (hgal) in follicular lymphomas比较的生发中心标记cd10 bcl6和人类胚center-associated淋巴瘤滤泡性淋巴瘤(hgal).pdfVIP

Goteri et al. Diagnostic Pathology 2011, 6:97 /content/6/1/97 RESEARCH Open Access Comparison of germinal center markers CD10, BCL6 and human germinal center-associated lymphoma (HGAL) in follicular lymphomas 1* 2 1 1 1 Gaia Goteri , Guendalina Lucarini , Antonio Zizzi , Antonello Costagliola , Federica Giantomassi , Daniela Stramazzotti1, Corrado Rubini1 and Pietro Leoni3 Abstract Background: Recently, human germinal center-associated lymphoma (HGAL) gene protein has been proposed as an adjunctive follicular marker to CD10 and BCL6. Methods: Our aim was to evaluate immunoreactivity for HGAL in 82 cases of follicular lymphomas (FLs) - 67 nodal, 5 cutaneous and 10 transformed - which were all analysed histologically, by immunohistochemistry and PCR. Results: Immunostaining for HGAL was more frequently positive (97.6%) than that for BCL6 (92.7%) and CD10 (90.2%) in FLs; the cases negative for bcl6 and/or for CD10 were all positive for HGAL, whereas the two cases negative for HGAL were positive with BCL6; no difference in HGAL immunostaining was found among different malignant subtypes or grades. Conclusions: Therefore, HGAL can be used in the immunostaining of FLs as the most sensitive germinal center (GC)-marker; when applied alone, it would half the immunostaining costs, reserving the use of the other two markers only to HGAL-negative cases. Virtual Slides: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/ vs/1255638215567329. Background marginal zone differentiation; less frequently they have a Follicular lymphoma (FL) is the commonest form of predominantly diffu