comparison of raw and processed radix polygoni multiflori (heshouwu) by high performance liquid chromatography and mass spectrometry比较原材料首乌(heshouwu)通过高效液相色谱和质谱分析.pdfVIP
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comparison of raw and processed radix polygoni multiflori (heshouwu) by high performance liquid chromatography and mass spectrometry比较原材料首乌(heshouwu)通过高效液相色谱和质谱分析
Liang et al. Chinese Medicine 2010, 5:29
/content/5/1/29
RESEARCH Open Access
Comparison of raw and processed Radix Polygoni
Multiflori (Heshouwu) by high performance liquid
chromatography and mass spectrometry
*
Zhitao Liang, Hubiao Chen, Zhiling Yu, Zhongzhen Zhao
Abstract
Background: Radix Polygoni Multiflori is the dried root tuber of Polygonum multiflorum Thunb. (Fam. Polygonaceae).
According to Chinese medicine theory, raw (R-RPM) and processed (P-RPM) Radix Polygoni Multiflori possess
different properties. The present study investigates the differences in chemistry between raw and processed Radix
Polygoni Multiflori.
Methods: Five pairs of R-RPM and P-RPM as well as 15 commercial decoction pieces were analyzed with high
performance liquid chromatography (HPLC) and mass spectrometry (MS).
Results: Two anthraquinones, namely emodin-8-O-(6′-O-malonyl)-glucoside and physcion-8-O-(6′-O-malonyl)-
glucoside disappeared or decreased significantly and 2,3,5,4′-tetrahydroxystilbene-2-O-b-D-glucopyranoside,
emodin-8-O-b-D-glucopyranoside and physcion-8-O-b-D-glucopyranoside decreased after the R-RPM samples being
processed. On the other hand, the contents of emodin and physcion generally increased after processing.
Conclusion: The present study indicates that processing Radix Polygoni Multiflori may change the contents and
types of chemicals in it. These changes are probably responsible for the various pharmacological effects of R-RPM
and P-RPM as well as hepatotoxicity.
Background vital essence and blood, blackens the hair and strength-
Proper pharmaceutical processing may reduce toxicity ens the tendons and bones.
or side effects, potentiate the
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