comprehensive analysis of nac domain transcription factor gene family in populus trichocarpa综合分析nac域转录因子的基因家族在杨树trichocarpa.pdfVIP

Hu et al. BMC Plant Biology 2010, 10:145 /1471-2229/10/145 RESEARCH ARTICLE Open Access Comprehensive Analysis of NAC Domain Transcription Factor Gene Family in Populus trichocarpa 1† 1† 1,2† 1 1 1* Ruibo Hu , Guang Qi , Yingzhen Kong , Dejing Kong , Qian Gao , Gongke Zhou Abstract Background: NAC (NAM, ATAF1/2 and CUC2) domain proteins are plant-specific transcriptional factors known to play diverse roles in various plant developmental processes. NAC transcription factors comprise of a large gene family represented by more than 100 members in Arabidopsis, rice and soybean etc. Recently, a preliminary phylogenetic analysis was reported for NAC gene family from 11 plant species. However, no comprehensive study incorporating phylogeny, chromosomal location, gene structure, conserved motifs, and expression profiling analysis has been presented thus far for the model tree species Populus. Results: In the present study, a comprehensive analysis of NAC gene family in Populus was performed. A total of 163 full-length NAC genes were identified in Populus, and they were phylogeneticly clustered into 18 distinct subfamilies. The gene structure and motif compositions were considerably conserved among the subfamilies. The distributions of 120 Populus NAC genes were non-random across the 19 linkage groups (LGs), and 87 genes (73%) were preferentially retained duplicates that located in both duplicated regions. The majority of NACs showed specific temporal and spatial expression patterns based on EST frequency and microarray data analyses. However, the expression patterns of a majority of duplicate genes were partially redundant, suggesting the occurrence of subfunctionalization during subsequent evolutionary process. Furthe