原创力文档computational modeling of the electrochemical system of lipase activity detection计算模型的脂肪酶活性检测的电化学系统.pdfVIP
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computational modeling of the electrochemical system of lipase activity detection计算模型的脂肪酶活性检测的电化学系统
Sensors 2008, 8, 3873-3879; DOI: 10.3390/s8063873
OPEN ACCESS
sensors
ISSN 1424-8220
/sensors
Article
Computational Modeling of the Electrochemical System of
Lipase Activity Detection
Mantas Puida 1,*, Feliksas Ivanauskas 1, Ilja Ignatjev 2, Gintaras Valinčius 2 and
2
Valdemaras Razumas
1 Faculty of Mathematics and Informatics, Vilnius University, Naugarduko 24, LT-03225 Vilnius,
Lithuania
2 Institute of Biochemistry, Mokslininkų 12, LT-08662 Vilnius, Lithuania
* Author to whom correspondence should be addressed: E-mail: mantasp@ (M.P.)
Received: 15 May 2008; in revised form: 31 May 2008 / Accepted: 1 June 2008 /
Published: 9 June 2008
Abstract: This paper presents computational modeling of response kinetics of
bioelectroanalytical system based on solid supported lipase substrate and lipase interaction.
The model assumes that lipase substrate is formed by dripping and drying a small amount
of the ethanol solution of 9-(5’-ferrocenylpentanoyloxy)nonyl disulfide (FPONDS) and that
lipase is capable of cleaving FPONDS ester bonds via hydrolysis mechanism. Two
mathematical models have been developed and evaluated trough computational simulation
series by comparing them to experimental data. The results of simulation demonstrate that
a good fitting might be obtained only taking into account non-linear substrate wash off
process.
Keywords: Amperometric biosensor, lipase activity, ferrocene-based substrate,
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