a global metabolic shift is linked to salmonella multicellular development一个全球沙门氏菌多细胞代谢改变与发展.pdfVIP

A Global Metabolic Shift Is Linked to Salmonella Multicellular Development 1¤a 2 1¤b 2 2 Aaron P. White , Aalim M. Weljie , Dmitry Apel , Ping Zhang , Rustem Shaykhutdinov , Hans J. 2 1 Vogel , Michael G. Surette * 1 Department of Microbiology and Infectious Diseases, University of Calgary, Calgary, Canada, 2 Department of Biological Sciences, University of Calgary, Calgary, Canada Abstract Bacteria can elaborate complex patterns of development that are dictated by temporally ordered patterns of gene expression, typically under the control of a master regulatory pathway. For some processes, such as biofilm development, regulators that initiate the process have been identified but subsequent phenotypic changes such as stress tolerance do not seem to be under the control of these same regulators. A hallmark feature of biofilms is growth within a self-produced extracellular matrix. In this study we used metabolomics to compare Salmonella cells in rdar colony biofilms to isogenic csgD deletion mutants that do not produce an extracellular matrix. The two populations show distinct metabolite profiles. Even though CsgD controls only extracellular matrix production, metabolite signatures associated with cellular adaptations associated with stress tolerances were present in the wild type but not the mutant cells. To further explore these differences we examine the temporal gene expression of genes implicated in biofilm development and stress adaptations. In wild type cells, genes involved in a metabolic shift to gluconeogenesis and various stress-resistance pathways exhibited an ordered expression profile timed with multicellular developme