a role for tlr4 in clostridium difficile infection and the recognition of surface layer proteins梭状芽胞杆菌的角色tlr4在感染和表层蛋白质的识别.pdfVIP

A Role for TLR4 in Clostridium difficile Infection and the Recognition of Surface Layer Proteins 1 1 2 2 2 3 Anthony Ryan , Mark Lynch , Sinead M. Smith , Sylvie Amu , Hendrik J. Nel , Claire E. McCoy , 1 1 1 1 2 ´ ´ 2 Jennifer K. Dowling , Eve Draper , Vincent O’Reilly , Ciara McCarthy , Julie O’Brien , Deirdre Nı Eidhin , Mary J. O’Connell4, Brian Keogh2, Charles O. Morton5, Thomas R. Rogers5, Padraic G. Fallon2, Luke A. O’Neill3, Dermot Kelleher2., Christine E. Loscher 1*. 1 Immunomodulation Research Group, School of Biotechnology, Dublin City University, Ireland, 2 Department of Clinical Medicine and Institute of Molecular Medicine, Trinity Centre for Health Sciences, Trinity College Dublin, St James’s Hospital, Dublin, Ireland, 3 School of Biochemistry and Immunology, Trinity College, Dublin, Ireland, 4 Molecular Evolution Group, School of Biotechnology, Dublin City University, Ireland, 5 Department of Clinical Microbiology, St James Hospital, Trinity College, Dublin, Ireland Abstract Clostridium difficile is the etiological agent of antibiotic-associated diarrhoea (AAD) and pseudomembranous colitis in humans. The role of the surface layer proteins (SLPs) in this disease has not yet been fully explored. The aim of this study was to investigate a role for SLPs in the recognition of C. difficile and the subsequent activation of the immune system. Bone marrow derived dendritic cells (DCs) exposed to SLPs were assessed for production of inflammatory cytokines, expression of c