action mechanisms of lithium chloride on cell infection by transmissible gastroenteritis coronavirus作用机制的氯化锂电池由传染性肠胃炎冠状病毒感染.pdfVIP

Action Mechanisms of Lithium Chloride on Cell Infection by Transmissible Gastroenteritis Coronavirus 1 1 2 1 1 1 3 Xiaofeng Ren *, Fandan Meng , Jiechao Yin , Guangxing Li , Xunliang Li , Chao Wang , Georg Herrler ¨ 1 College of Veterinary Medicine, Northeast Agricultural University, Harbin, China, 2 College of Life Sciences, Northeast Agricultural University, Harbin, China, 3 Institut fur ¨ Virologie, Tierarztliche Hochschule Hannover, Hannover, Germany Abstract Transmissible gastroenteritis virus (TGEV) is a porcine coronavirus. Lithium chloride (LiCl) has been found to be effective against several DNA viruses, such as Herpes simplex virus and vaccinia virus. Recently, we and others have reported the inhibitory effect of LiCl on avian infectious bronchitis coronavirus (IBV) infection, an RNA virus. In the current study, the action mechanism of LiCl on cell infection by TGEV was investigated. Plaque assays and 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5- di-phenyl tetrazoliumbromide (MTT) assays showed that the cell infection by TGEV was inhibited in a dose-dependent manner, when LiCl was added to virus-infected cells; the cell infection was not affected when either cells or viruses were pretreated with the drug. The inhibition of TGEV infection in vitro by LiCl was observed at different virus doses and with different cell lines. The inhibitory effect of LiCl against TGEV infection and transcription was confirmed by RT-PCR and real- time PCR targeting viral S and 3CL-protease genes. The time-of-addition effect of the drug on TGEV infection indic