原创力文档age-dependent changes in the proteome following complete spinal cord transection in a postnatal south american opossum (monodelphis domestica)年龄相关性变化的蛋白质组完成脊髓横断后产后南美负鼠(把).pdfVIP
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age-dependent changes in the proteome following complete spinal cord transection in a postnatal south american opossum (monodelphis domestica)年龄相关性变化的蛋白质组完成脊髓横断后产后南美负鼠(把)
Age-Dependent Changes in the Proteome Following
Complete Spinal Cord Transection in a Postnatal South
American Opossum (Monodelphis domestica)
1 2 1 1 3
Natassya M. Noor , David L. Steer , Benjamin J. Wheaton , C. Joakim Ek , Jessie S. Truettner , W. Dalton
3 1 4 2 5
Dietrich , Katarzyna M. Dziegielewska , Samantha J. Richardson , A. Ian Smith , John L. VandeBerg ,
Norman R. Saunders1*
1 Department of Pharmacology, the University of Melbourne, Parkville, Victoria, Australia, 2 Department of Biochemistry Molecular Biology, Monash University, Clayton,
Victoria, Australia, 3 The Miami Project to Cure Paralysis, University of Miami, Miller School of Medicine, Miami, Florida, United States of America, 4 School of Medical
Sciences and Health Innovations Research Institute, RMIT University, Bundoora, Victoria, Australia, 5 Texas Biomedical Research Institute, San Antonio, Texas, United States
of America
Abstract
Recovery from severe spinal injury in adults is limited, compared to immature animals who demonstrate some capacity for
repair. Using laboratory opossums (Monodelphis domestica), the aim was to compare proteomic responses to injury at two
ages: one when there is axonal growth across the lesion and substantial behavioural recovery and one when no axonal
growth occurs. Anaesthetized pups at postnatal day (P) 7 or P28 were subjected to complete transection of the spinal cord
at thoracic level T10. Cords were collected 1 or 7 days after injury and from age-matched controls. Proteins were separated
based on isoelectric point and subunit molecular weight; those whose expression levels changed following injury we
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