原创力文档altered expression of porcine piwi genes and pirna during development改变猪匹威基因的表达和pirna发展.pdfVIP
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altered expression of porcine piwi genes and pirna during development改变猪匹威基因的表达和pirna发展
Altered Expression of Porcine Piwi Genes and piRNA
during Development
1 2 2 1
Dorota Kowalczykiewicz , Piotr Pawlak , Dorota Lechniak , Jan Wrzesinski *
´ ´
1 Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland, 2 Department of Genetics and Animal Breeding, University of Life Sciences, Poznan,
Poland
Abstract
Three Sus scrofa Piwi genes (Piwil1, Piwil2 and Piwil4) encoding proteins of 861, 985 and 853 aminoacids, respectively, were
cloned and sequenced. Alignment of the Piwi proteins showed the high identity between Sus scrofa and Homo sapiens.
Relative transcript abundance of porcine Piwil1, Piwil2 and Piwil4 genes in testes, ovaries and oocytes derived from sexually
immature and mature animals was examined using Real-Time PCR. Expression of the three Piwi mRNAs was proved to be
tissue specific and restricted exclusively to the gonads. In testes of adult pigs the highest relative transcript abundance was
observed for the Sus scrofa Piwil1 gene. On the other hand, in testes of neonatal pigs the Piwil1 transcript level was over 2–
fold reduced while the level of Piwil2 transcript was higher. As regards the expression of the Piwil4 transcript, its level was
34-fold elevated in testes of neonatal piglet when compared to adult male. In ovaries of prepubertal and pubertal female
pigs transcript abundance of the three Piwi genes was significantly reduced in comparison with testes. However, similarly to
testes, in ovaries of neonatal pigs the Piwil2 gene was characterized by the highest relative transcript abundance among the
three Piwi genes analysed. In prepubertal and pubertal oocyte
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