altered trabecular bone structure and delayed cartilage degeneration in the knees of collagen vi null mice改变骨小梁结构和延迟的膝盖软骨变性胶原vi零老鼠.pdfVIP

Altered Trabecular Bone Structure and Delayed Cartilage Degeneration in the Knees of Collagen VI Null Mice 1,2 3 1 1 1 Susan E. Christensen , Jeffrey M. Coles , Nicole A. Zelenski , Bridgette D. Furman , Holly A. Leddy , 3 4 1,2,3 Stefan Zauscher , Paolo Bonaldo , Farshid Guilak * 1 Department of Orthopaedic Surgery, Duke University Medical Center, Durham, North Carolina, United States of America, 2 Department of Biomedical Engineering, Duke University, Durham, North Carolina, United States of America, 3 Department of Mechanical Engineering Materials Science, Duke University, Durham, North Carolina, United States of America, 4 Department of Histology, Microbiology and Medical Biotechnologies, University of Padova, Padova, Italy Abstract Mutation or loss of collagen VI has been linked to a variety of musculoskeletal abnormalities, particularly muscular dystrophies, tissue ossification and/or fibrosis, and hip osteoarthritis. However, the role of collagen VI in bone and cartilage structure and function in the knee is unknown. In this study, we examined the role of collagen VI in the morphology and physical properties of bone and cartilage in the knee joint of Col6a12/ 2 mice by micro-computed tomography (microCT), histology, atomic force microscopy (AFM), and scanning microphotolysis (SCAMP). Col6a12/ 2 mice showed significant differences in trabecular bone structure, with lower bone volume, connectivity density, trabecular number, and trabecular thickness but higher structure model index and trabecular separation compared to Col6a1+/+ mi