antibody-antigen-adjuvant conjugates enable co-delivery of antigen and adjuvant to dendritic cells in cis but only have partial targeting specificityantibody-antigen-adjuvant配合使co-delivery树突细胞的抗原和佐剂cis但是只有部分针对特异性.pdfVIP

Antibody-Antigen-Adjuvant Conjugates Enable Co- Delivery of Antigen and Adjuvant to Dendritic Cells in Cis but Only Have Partial Targeting Specificity 1,2. 1. 1. 3 4 4 Martin Kreutz , Benoit Giquel , Qin Hu , Ram Abuknesha , Satoshi Uematsu , Shizuo Akira , 5 1 Frank O. Nestle , Sandra S. Diebold * 1 Peter Gorer Department of Immunobiology, King’s College London, Guy’s Hospital, London, United Kingdom, 2 Department of Tumor Immunology, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands, 3 Pharmaceutical Science Division, King’s College London, London, United Kingdom, 4 Laboratory of Host Defense, WPI Immunology Frontier Research Center, Osaka University, Osaka, Japan, 5 Cutaneous Medicine and Immunotherapy Unit, King’s College London, Guy’s Hospital, London, United Kingdom Abstract Antibody-antigen conjugates, which promote antigen-presentation by dendritic cells (DC) by means of targeted delivery of antigen to particular DC subsets, represent a powerful vaccination approach. To ensure immunity rather than tolerance induction the co-administration of a suitable adjuvant is paramount. However, co-administration of unlinked adjuvant cannot ensure that all cells targeted by the antibody conjugates are appropriately activated. Furthermore, antigen- presenting cells (APC) that do not present the desired antigen are equally strongly activated and could prime undesired responses against self-antigens. We, therefore, were interested in exploring targeted co-delivery of antigen and adjuvant in cis in form of antibody-antigen-adjuvant conjugates for the induction of