ap-1 transcription factors, mucin-type molecules and mmps regulate the il-11 mediated invasiveness of jeg-3 and htr-8svneo trophoblastic cellsap-1转录因子,mucin-type分子和基质金属蛋白酶调节il-11介导jeg-3和htr-8svneo滋养层细胞的侵袭性.pdfVIP

AP-1 Transcription Factors, Mucin-Type Molecules and MMPs Regulate the IL-11 Mediated Invasiveness of JEG-3 and HTR-8/SVneo Trophoblastic Cells 1 2 1 3 2 Pankaj Suman , Geeta Godbole , Ravi Thakur , Diana M. Morales-Prieto , Deepak N. Modi , Udo R. 3 1 Markert , Satish K. Gupta * 1 Reproductive Cell Biology Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, India, 2 National Institute for Research in Reproductive Health, Jehangir Merwanji Street, Parel, Mumbai, India, 3 Placenta Laboratory, Department of Obstetrics, Faculty of Medicine, Friedrich-Schiller University, Jena, Germany Abstract This study examines the IL-11 mediated activation of downstream signaling and expression of effector molecules to resolve the controversies associated with the IL-11 mediated regulation of the invasiveness of two commonly used trophoblastic cell models viz. JEG-3 and HTR-8/SVneo cells. It has been reported that IL-11 increases the invasiveness of JEG-3 cells while, reduces the invasiveness of HTR-8/SVneo cells. Invasion assay performed simultaneously for both the cell lines confirmed the above findings. In addition, HTR-8/SVneo cells showed a higher basal invasiveness than JEG-3 cells. Western blot showed the IL-11 mediated activation of STAT3(tyr705) and STAT1(tyr701) in both the cell lines. However, IL-11 activated the ERK1/2 phosphorylation in JEG-3 cells but, inhibited it in HTR-8/SVneo cells. Within 10 min of IL-11 treatment, p- STAT3(tyr705) was localized inside the nucleus of both the cell lines but, there was enhanced co-localization of protein inhibitor of activated STAT1/3 (PIAS1/3) and p-STAT3(tyr705) in HTR-8/SVneo c