arabidopsis bhlh100 and bhlh101 control iron homeostasis via a fit-independent pathway拟南芥bhlh100通过fit-independent通路和bhlh101控制铁体内平衡.pdfVIP

Arabidopsis bHLH100 and bHLH101 Control Iron Homeostasis via a FIT-Independent Pathway 1,2 1 1 ´ 1,3 Alicia B. Sivitz , Victor Hermand , Catherine Curie , Gregory Vert * ´ ´ ´ 1 Biochimie et Physiologie Moleculaire des Plantes, Centre National de la Recherche Scientifique Unite Mixte de Recherche 5004, Institut de Biologie Integrative des Plantes, Montpellier, France, 2 Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire, United States of America, 3 Plant Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California, United States of America Abstract Iron deficiency induces a complex set of responses in plants, including developmental and physiological changes, to increase iron uptake from soil. In Arabidopsis, many transporters involved in the absorption and distribution of iron have been identified over the past decade. However, little is known about the signaling pathways and networks driving the various responses to low iron. Only the basic helix–loop–helix (bHLH) transcription factor FIT has been shown to control the expression of the root iron uptake machinery genes FRO2 and IRT1. Here, we characterize the biological role of two other iron-regulated transcription factors, bHLH100 and bHLH101, in iron homeostasis. First direct transcriptional targets of FIT were determined in vivo. We show that bHLH100 and bHLH101 do not regulate FIT target genes, suggesting that they play a non-redundant role with the two closely related bHLH factors bHLH038 and bHLH039 that have been suggested to act in concert with