arp23 complex regulates asymmetric division and cytokinesis in mouse oocytesarp23复杂的调节在小鼠卵母细胞不对称分裂和胞质分裂.pdfVIP

Arp2/3 Complex Regulates Asymmetric Division and Cytokinesis in Mouse Oocytes 1 2 1 1 1 1 Shao-Chen Sun , Zhen-Bo Wang , Yong-Nan Xu , Seung-Eun Lee , Xiang-Shun Cui , Nam-Hyung Kim * 1 Department of Animal Sciences, Chungbuk National University, Cheongju, Republic of Korea, 2 State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China Abstract Mammalian oocyte meiotic maturation involves oocyte polarization and a unique asymmetric division, but until now, the underlying mechanisms have been poorly understood. Arp2/3 complex has been shown to regulate actin nucleation and is widely involved in a diverse range of processes such as cell locomotion, phagocytosis and the establishment of cell polarity. Whether Arp2/3 complex participates in oocyte polarization and asymmetric division is unknown. The present study investigated the expression and functions of Arp2/3 complex during mouse oocyte meiotic maturation. Immunofluorescent staining showed that the Arp2/3 complex was restricted to the cortex, with a thickened cap above the meiotic apparatus, and that this localization pattern was depended on actin. Disruption of Arp2/3 complex by a newly-found specific inhibitor CK666, as well as by Arpc2 and Arpc3 RNAi, resulted in a range of effects. These included the failure of asymmetric division, spindle migration, and the formation and completion of oocyte cytokinesis. The formation of the actin cap and cortical granule-free domain (CGFD) was also disrupted, which further confirmed the disruption of spindle migration. Our data suggest that the Arp2/3 complex probably regulates oocyte polarization through